Human eccrine sweat contains numerous biomarkers which can provide information on health,

performance, and aging. Non-invasive collection and measurement of biomarkers has become

especially important in recent times given viral outbreaks like SARS-CoV-2. In the current study

we describe a method of sweat collection from palmar surfaces in participants via surface capture

using glass beads and the resulting analysis of biomarkers from very low volumes of sweat using

liquid chromatography mass spectrometry with selected ion monitoring. Study participants

underwent a cognitive and physical stress task with easy and hard conditions with sweat being

collected after each task. Resulting analysis found a signal for 22 steroid biomarkers and we

report detailed information on selected biomarkers, given their applicability to timely real-world

exemplars, including cortisol, dehydroepiandrosterone, allopregnanolone, estrone, aldosterone,

and 20a/b-dihydrocortisone.

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