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Reactions of cold argon plasmas with condensed-phase peptides and proteins for mass spectrometry imaging and structural elucidation

06 November 2024, Version 2
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

The analysis of macromolecules - such as proteins - pose a significant bottleneck in analytical science. We have developed surface treatment techniques utilising direct and radiofrequency current argon plasmas, able to quickly digest condensed-phase macromolecules while maintaining spatial resolution of analytes. We show here that these plasmas can be powerful tools for mass spectrometry imaging of peptides and proteins.

Keywords

Plasma
Mass Spectrometry Imaging
Spatial Resolution
Proteomics
Peptidomics
Macromolecules

Supplementary materials

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Methodology, data, and images for 'Reactions of cold argon plasmas with condensed-phase peptides and proteins for mass spectrometry imaging and structural elucidation'
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Version History

Nov 06, 2024 Version 2

Version Notes

The paper has been reworked based on feedback from reviewers, incorporating additional data (e.g demonstrating the spatial resolution improvements associated with the plasma compared to trypsin), adding some graphs, changing some nomenclature, and amending some sentences.

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The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2024-sjwlb-v2
D O I: 10.26434/chemrxiv-2024-sjwlb-v2 [opens in a new tab]

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) declare that they have sought and gained approval from the relevant ethics committee/IRB for this research and its publication.

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