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Transfection of Macrophages with PAMAM-Pyrrolidone Dendrimers Complexed with Cu(II)- or Zn(II)- ions as Carriers of Genetic Material.

11 September 2024, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Zn(II) and Cu(II) complexes of a generation 4 DAB-core PAMAM dendrimer with 5-methoxycarbonyl-2-pyrrolidinone (Pyrr) surface groups were prepared and investigated as carriers for siRNA in different cell lines. The Cu(II) complex (CuSO4)18@DAB-dendr-(Pyrr)64 is significantly better than lipofectamine for transfection of macrophages, while the Zn(II) complex only show low transfection activity. Both complexes are more cytotoxic than the free metal ions.

Keywords

Dendrimers
in vitro transfection
macrophages
metal complexation
Zinc(II)
Copper(II).

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Version History

Sep 11, 2024 Version 1

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The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2024-ltgwp
D O I: 10.26434/chemrxiv-2024-ltgwp [opens in a new tab]

Funding

COST Action CA17140

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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