viAFM - a Python toolset for virtual Atomic Force Microscopy

Atomic Force Microscopy (AFM) is a powerful but demanding imaging technique used also to study the interaction strength of single biomolecules by force spectroscopy. Here we provide a viAFM program designed to facilitate multi- directional steered molecular dynamics simulations and analysis of forces required to disrupt protein complexes thus mimicking AFM force spectroscopy experiments. Our viAFM tool allows for rapid assessment of protein-protein interaction strength, anisotropy, and the impact of ligands or mutations on complex stability, enabling efficient testing of drug candidates.