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Abstract
Large- to ultra-large-scale synthesis of nucleic acids is becoming an increasingly important tool for understanding and manipulating biological systems, as well as for developing new technologies based on engineered biological materials, including DNA-based nanofabrication, aptamers and writing digital data at the molecular level. Several technologies for large-scale synthesis have been developed over the years, but all of them remain inaccessible to most researchers due to their complexity and/or use of proprietary technologies. Here, we present a fully open source, benchtop device for ultra-large scale nucleic acid synthesis that is also highly flexible and adaptable, able to accommodate a wide range of monomers and chemistries, while providing unrestricted access to synthesis parameter space.
Supplementary materials
Title
Mechanical drawings MAS 2.0
Description
Mechanical drawings of all of the components of the MAS 2.0 in pdf format (82 files)
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Title
Optical drawings MAS 2.0
Description
Optical drawings for MAS 2.0 in pdf format (6 files)
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Step files of all the mechanical components of the MAS 2.0 (86 files))
Description
CAD files of all the mechanical components of the MAS 2.0 in step format (.stp). Includes assembly tools. 87 files.
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Title
STL files for components of the MAS 2.0 to be 3D printed (3 files)
Description
STL files for components to be 3D printed
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Title
Component list spreadsheet MAS 2.0
Description
Spreadsheet with a list components for MAS 2.0.
Tab 1. Machined metal components (including cost estimate)
Tab 2. Plastic 3D components
Tab 3. List of fasteners and other hardware parts
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Title
MAS 2.0 Photolithographic Synthesis of Nucleic Acids Process and Reference Manual
Description
A detailed manual on the use of MAS 2.0 for the large-scale synthesis of nucleic acids. Incudes background material on the chemistry and photochemistry as well as usage instructions, procedures and protocols for nucleic acid synthesis and post-processing. Also includes list of reagents, solvents and other materials (electronics, optics, optomechanics, fluidics, and their commercial sources. Includes information on fluidics protocols and software usage instructions. References to relevant background publications in the scientific literature are also included.
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Title
CAD files for MAS 2.0 assembly
Description
Fusion 360 archive file of MAS 2.0 assembly
Step file of MAS 2.0 assembly exported from Fusion 360
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Title
Python software for MAS 2.0
Description
Software for running the MAS 2.0 device with either a XGA or 1080p DLP modules (current hardware versions, as well as DLLs from Vialux). The software is also configured for use with a LabJack U12 DAQ, an Expedite 8909 nucleic acid synthesizer, and with temperature and humidity logging with a W&T Web-Thermo-Hygrometer. User interface instruction are provided in the MAS 2.0 Photolithographic Synthesis of Nucleic Acids Process and Reference Manual.
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Supplementary weblinks
Title
Viewable and downloadable Fusion 360 assembly of MAS 2.0
Description
This is a link to a Fusion 360 viewer at myhub.autodesk360.com. The link shows the CAD assembly of the MAS 2.0 system. The design can be explored but not edited. It can be downloaded and opened in Fusion 360 with full access if you have a Fusion 360 account, or alternative download formats are available for common CAD file types. The downloaded version is the same as that provided in the supplementary material titled CAD files for MAS 2.0 assembly.
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