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Abstract
We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect® tubes) and corresponding venous blood (10 mL vacutainers). Samples from twenty healthy participants were analysed by 1H-NMR and 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters from SPC1, SPC2, and SPC3 subfractions; 2 N-acetyl signals from α-1-acid glycoprotein (Glyc), GlycA and GlycB; and 3 calculated parameters, SPC (total), SPC3/SPC2, Glyc (total)—were assessed. Using linear regression between capillary and venous collection sites, explained variance (Adj. R2 ≥ 0.8, p < 0.001) was witnessed for 86% of plasma parameters (103/120), and 88% of serum parameters (106/120), indicating capillary lipoprotein, SPC, and Glyc concentration follows changes in venous concentra-tions. These results indicate capillary blood microsamples are suitable for sampling in remote areas and for high-frequency longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.
Supplementary materials
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Supporting Information
Description
Table S1. Annotated List of Lipoproteins, SPCs, and Glycs Derived From B.I. LISATM and PhenoRisk PACSTM RuO Models with Calculated Means and Range by Collection Site and Biofluid
Figure S1. Direct Low-Field 80MHz JEDI-PGSE Proton NMR Spectra. 1D (A) and JEDI - PGSE (B) NMR spectra of capillary and venous plasma samples collected from two participants (participant 1 = left figures, participant 2 = right figures). Capillary spectra (green trace) is overlaid with venous (black trace) derived from the 1D and PGSE experiments. Overlap is demonstrated between capillary and venous plasma samples for SPC and Glyc measurements.
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