The Utility of Thiocarbohydrazide for Generating Novel Triazole and Pyrazole Derivatives Containing a Sulfur Moiety as Anti-microbial Agents

A crucial class of chemicals with uses in numerous fields is the thiocarbohydrazide class. This study aims to investigate the efficiency of thiocarbohydrazide (1) for creating innovative triazole and pyrazole derivatives that include a sulfur moiety with prospective biological activity, as the chemistry of thiocarbohydrazides has drawn increased interest in both synthetic organic chemistry and biological domains. When thiocarbohydrazide (1) intereacts with carboxylic acids 2a-c (stearic acid, gallic acid and 4-hydrobenzoic acid) afforded the corresponding 4H-1,2,4-triazole deivatives 3a-c, which ultimately underwent a reaction with salicylic acid 4, chloroacetic acid 6, 2-bromo-1,3-diphenylpropane-1,3-dione (9), ethyl cyanoacetate, furan-2-carbaldehyde (15) and acetylacetone 17 to afford the respective triazolo[3,4-b][1,3,4]thiadiazole derivative 5b, 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine derivatives 8a-c, 11a-c, 13, 5-(furan-2-yl)-4H-1,2,4-triazole-3-thiol (16) and 3,5-dimethyl-1H-pyrazole-1-carbothiohydrazide (18). Each constructed product's molecular structure was reinforced by executing IR, 1H NMR, and mass spectrometry techniques. Some of the constructed chemicals' anti-bacterial and anti-fungal effectiveness was also evaluated against Staphylococcus aureus ATCC 25923 (Gram Positive Bacteria), Proteus vulgaris RCMB 004 (1) ATCC 13315 (Gram Negative Bacteria), Aspergillus fumigatus (RCMB 002008) and Candida albicans RCMB 005003 (1) ATCC 10231 (Fungi). According to these research findings, some of the examined compounds have promising antimicrobial properties.