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Abstract
Preventing fluorophore photobleaching and unwanted blinking is crucial for singlemolecule
fluorescence studies. Reductants achieve photoprotection via quenching excited tripletstates,
yet either require counteragents, or for popular alkyl-thiols, are limited to cyanine dye Cy3
protection. Here we provide mechanistic and imaging results showing that the naturally occurring
amino acid ergothioneine and its analogue dramatically enhance photostability for Cy3, Cy5 and
their conformationally restrained congeners, providing a biocompatible universal solution for
demanding fluorescence imaging.
Supplementary materials
Title
Supporting Information for: Superior Photoprotection of Cyanine Dyes with Thio-imidazole Amino Acids
Description
The supplementary material contains a materials and methods section describing the performed experiments as well as additional figures and tables.
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