Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

Selectivity and Efficiency of the Ligation of The Pyrene:Abasic Base Pair by T4 DNA Ligase: Towards Abasic Lesion Detection by Ligation

10 June 2022, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Remarkable selectivity was observed in the ligation of 5’-phosphate 1-pyrene nucleotide terminated strands across from an abasic lesion in a DNA-templated ligation reaction by two different ligase suggesting that pyrene-terminated strands could be used in abasic site detection. This selectivity is significantly greater than other reported unnatural base pairs at the same position revealing the unique properties of the abasic:pyrene pair.

Keywords

unnatural base
ligase
abasic detection

Supplementary materials

Title
Description
Actions
Title
Supporting Information of Selectivity and Efficiency of the Ligation of The Pyrene:Abasic Base Pair by T4 DNA Ligase: Towards Abasic Lesion Detection by Ligation
Description
Electronic Supporting Information (ESI) of manuscript "Selectivity and Efficiency of the Ligation of The Pyrene:Abasic Base Pair by T4 DNA Ligase: Towards Abasic Lesion Detection by Ligation"
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Now Published

Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases [opens in a new tab]
Hansol Park, Julianne M. Gibbs journal article
Chemical Communications , Volume 58, Issue 65
Online publication date: 2022

Version History

Jun 10, 2022 Version 1

Metrics

428
251
0
Views
Downloads
Citations

License

CC logo
CC
BY logo
BY
NC logo
NC
ND logo
ND
The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2022-f529m
D O I: 10.26434/chemrxiv-2022-f529m [opens in a new tab]

Funding

Natural Sciences and Engineering Research Council of Canada
CREATE-463990-2015
Natural Sciences and Engineering Research Council of Canada
RGPIN-2020-05976

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

Share