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Selectivity and Efficiency of the Ligation of The Pyrene:Abasic Base Pair by T4 DNA Ligase: Towards Abasic Lesion Detection by Ligation

10 June 2022, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Remarkable selectivity was observed in the ligation of 5’-phosphate 1-pyrene nucleotide terminated strands across from an abasic lesion in a DNA-templated ligation reaction by two different ligase suggesting that pyrene-terminated strands could be used in abasic site detection. This selectivity is significantly greater than other reported unnatural base pairs at the same position revealing the unique properties of the abasic:pyrene pair.

Keywords

unnatural base
ligase
abasic detection

Supplementary materials

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Description
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Title
Supporting Information of Selectivity and Efficiency of the Ligation of The Pyrene:Abasic Base Pair by T4 DNA Ligase: Towards Abasic Lesion Detection by Ligation
Description
Electronic Supporting Information (ESI) of manuscript "Selectivity and Efficiency of the Ligation of The Pyrene:Abasic Base Pair by T4 DNA Ligase: Towards Abasic Lesion Detection by Ligation"
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Now Published

Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases [opens in a new tab]
Hansol Park, Julianne M. Gibbs journal article
Chemical Communications , Volume 58, Issue 65
Online publication date: 2022

Version History

Jun 10, 2022 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2022-f529m
D O I: 10.26434/chemrxiv-2022-f529m [opens in a new tab]

Funding

Natural Sciences and Engineering Research Council of Canada
CREATE-463990-2015
Natural Sciences and Engineering Research Council of Canada
RGPIN-2020-05976

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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