Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

Organoarsenic probes to study proteins by NMR spectroscopy

29 November 2021, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Arsenical probes enable structural studies of proteins. We report the first organoarsenic probes for nuclear magnetic resonance (NMR) spectroscopy to study proteins in solutions. These probes can be attached to irregular loop regions. A lanthanide-binding tag induces sizable pseudocontact shifts in protein NMR spectra of a magnitude never observed for small paramagnetic probes before.

Supplementary materials

Title
Description
Actions
Title
Supporting Information
Description
Detailed procedures and conditions for probe synthesis, protein expression and purification, sample preparation; tables of PCSs and fitted tensor parameters; correlations between back-calculated and measured PCSs; mass spectrometry data; EPR data.
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Now Published

Organoarsenic probes to study proteins by NMR spectroscopy [opens in a new tab]
Mithun C. Mahawaththa, Henry W. Orton, Ibidolapo Adekoya, Thomas Huber, Gottfried Otting, Christoph Nitsche journal article
Chemical Communications , Volume 58, Issue 5
Online publication date: 2022

Version History

Nov 29, 2021 Version 1

Metrics

639
564
0
Views
Downloads
Citations

License

CC logo
CC
BY logo
BY
NC logo
NC
ND logo
ND
The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2021-48dn4
D O I: 10.26434/chemrxiv-2021-48dn4 [opens in a new tab]

Funding

Australian Research Council
DE190100015; DP200100348; FL170100019; CE200100012
Alexander von Humboldt-Stiftung
Feodor Lynen Research Fellowship

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

Share