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Enhancing The Photocatalytic Conversion Of Pt(IV) Substrates By Flavoprotein Engineering

08 February 2021, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site directed mutagenesis on the mini Singlet Oxygen Generator (mSOG) to modulate the photocatalytic activity of this flavoprotein towards two model Pt(IV) substrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic efficiency as a result of its longer triplet excited state lifetime. This study shows, for the first time, that protein engineering can improve the catalytic capacity of a protein towards metal-containing substrate.

Keywords

Bioinorganic Chemical Reactivity
photocatalysis
Metal Drugs
flavoproteins
Protein engineering

Supplementary materials

Title
Description
Actions
Title
Gurruchaga mSOG SI ChemRxiv
Description
Actions

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Version History

Feb 08, 2021 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.13701994.v1
D O I: 10.26434/chemrxiv.13701994.v1 [opens in a new tab]

Funding

Eusko Jaurlaritza IT1254-19 and IT912-16
ERC-CoG-648071-ProNANO
Maria de Maeztu Units of Excellence Program of the Spanish State Research Agency - MDM-2017-0720
Severo Ochoa Centres of Excellence Program of the Spanish State Research Agency CEX2018-000867-S
Ministerio de Ciencia e Innovación, grants ERACoBioTech HOMBIOCAT-PCI2018-092984, MAT2017-83856-C3-3-P, PGC2018-097529-B-100, PID2019-109111RB-I00, PID2019-111649RB-I00, RED2018-102471-T

Author’s competing interest statement

No conflicts of interest

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