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SP3-FAIMS Chemoproteomics for High Coverage Profiling of the Human Cysteinome

28 December 2020, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We report a new cysteine chemoproteomic method, termed SP3-FAIMS chemoproteomics, which enables rapid and high coverage analysis of the human cysteinome. By combining enhanced cysteine biotinylation with SP3 sample decontamination and FAIMS online fraction, we identified in aggregate 34,225 unique cysteines found on 7,243 proteins. Showcasing the versatility of our method, integration with the isoTOP-ABPP workflow enabled the high throughput discovery of cysteines labelled by electrophilic compounds.

Keywords

Cysteine
Chemoproteomics
SP3
FAIMS
LC-MS/MS: liquid chromatography–tandem mass spectrometry

Supplementary materials

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Version History

Dec 28, 2020 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.13487364.v1
D O I: 10.26434/chemrxiv.13487364.v1 [opens in a new tab]

Funding

Beckman Young Investigator Award
V Scholar Award V2019-017
TRDRP T31DT1800
Chemistry Biology Interface Training Program T32GM008496
NIGMS System and Integrative Biology 5T32GM008185-33

Author’s competing interest statement

No conflicts of interest

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