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RNA Phosphorothioate Modification in Prokaryotes and Eukaryotes

14 January 2020, Version 2
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We report the first RNA phosphorothioate modification in both prokaryotes and eukaryotes. The GpsG modification exists in the Rp configuration and was quantified with liquid chromatography coupled with tandem mass spectrometry. Furthermore, we show the Dnd clusters that regulate DNA PT modification also play roles in RNA PT modification.

Keywords

RNA
post-transcriptional modifications
Phosphorothioate

Supplementary materials

Title
Description
Actions
Title
PT Supplementary Information
Description
Actions
Title
Seq DndA knock out
Description
Actions

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Now Published

RNA Phosphorothioate Modification in Prokaryotes and Eukaryotes [opens in a new tab]
Ying Wu, Yaning Tang, Xiaolong Dong, Ya Ying Zheng, Phensinee Haruehanroengra, Song Mao, Qishan Lin, Jia Sheng journal article
ACS Chemical Biology , Volume 15, Issue 6
Online publication date: Apr 10, 2020

Version History

Jan 14, 2020 Version 2

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.11574099.v2
D O I: 10.26434/chemrxiv.11574099.v2 [opens in a new tab]

Funding

NSF-1845486

Author’s competing interest statement

The authors declare no conflict of interest.

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