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In Vitro Reconstitution and Kinetic Characterization of the Essential Condensation Enzymes Involved in Prodiginine and Tambjamine Biosynthesis

19 April 2019, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We have reconstituted the in vitro activity of the purified condensation enzymes PigC and TamQ, from prodiginine and tambjamine pathways respectively. Upon analysis and comparison of their kinetic profiles, a ping-pong reaction sequence with substrate inhibition was identified for each condensation enzyme.

Keywords

Natural Products Chemistry
Enzymology
Enzyme Mechanism

Supplementary materials

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Title
Picott 2019 Supplemental Information ChemRxiv
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Version History

Apr 19, 2019 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.8001410.v1
D O I: 10.26434/chemrxiv.8001410.v1 [opens in a new tab]

Author’s competing interest statement

No conflict of interest

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