Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

16 October 2018, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a relevant IC50 of 60 µM.


Keywords

Leishmania donovani
Polyamine transport
Radioisotopic method
fluorescence

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Version History

Oct 16, 2018 Version 1

Metrics

3,397
952
0
Views
Downloads
Citations

License

CC logo
CC
BY logo
BY
NC logo
NC
ND logo
ND
The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.7207448.v1
D O I: 10.26434/chemrxiv.7207448.v1 [opens in a new tab]

Funding

This work was supported by Initiative d’Excellence Paris-Saclay (IdEx)

Author’s competing interest statement

None

Share