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Spermine-NBD as Fluorescent Probe for Studies of Polyamine Transport System in Leishmania Donovani

16 October 2018, Version 1
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a relevant IC50 of 60 µM.


Keywords

Leishmania donovani
Polyamine transport
Radioisotopic method
fluorescence

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Version History

Oct 16, 2018 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.7207448.v1
D O I: 10.26434/chemrxiv.7207448.v1 [opens in a new tab]

Funding

This work was supported by Initiative d’Excellence Paris-Saclay (IdEx)

Author’s competing interest statement

None

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